Journal
/
Inmunología y contagio
/
Fecal (micro) RNA Isolation
JoVE Revista
Inmunología y contagio
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Inmunología y contagio
Fecal (micro) RNA Isolation

Fecal (micro) RNA Isolation

4,132 Views

•

05:35 min

•

October 28, 2020

DOI:

10.3791/61908-v

DOI:
10.3791/61908-v

•

05:35 min
October 28, 2020

•

42 Views
, ,
1Ann Romney Center for Neurologic Diseases, Department of Neurology, Partners Multiple Sclerosis Center,Brigham and Women’s Hospital and Harvard Medical School, 2Evergrande Center for Immunologic Diseases,Harvard Medical School and Brigham and Women’s Hospital

Summary

Automatically generated

This protocol isolates high quality total RNA from fecal samples of animal and human subjects. A commercial miRNA isolation kit is used with significant adaption to isolate pure RNA with optimized quantity and quality. The RNA isolates are good for most downstream RNA assays such as sequencing, micro-array, and RT-PCR.

Explore More Videos

FecalMicroRNARNA IsolationDPBSAcid Phenol ChloroformEthanolFilter CartridgeWash Solution

Read Article

Cite this Article

Dhang, F. H., Weiner, H. L., Liu, S. Fecal (micro) RNA Isolation. J. Vis. Exp. (164), e61908, doi:10.3791/61908 (2020).

Download .ris file

Copy

Share Video

.

Copy