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Avaliando o impacto da fratura hidráulica nos córregos usando assinaturas moleculares microbianas
JoVE Journal
Environment
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JoVE Journal Environment
Evaluating the Impact of Hydraulic Fracturing on Streams using Microbial Molecular Signatures

Avaliando o impacto da fratura hidráulica nos córregos usando assinaturas moleculares microbianas

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2,868 Views

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09:11 min

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April 04, 2021

DOI:

10.3791/61904-v

DOI:
10.3791/61904-v

•

09:11 min
April 04, 2021

•

5 Views
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1Department of Biology,Juniata College, 2Wright Labs, LLC, 3Department of Biological Sciences,Michigan Technological University, 4Biosciences Division,Oak Ridge National Laboratory, 5Department of Civil and Environmental Engineering,University of Tennessee

Summary

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Aqui, apresentamos um protocolo para investigar os impactos da fratura hidráulica nos córregos próximos, analisando suas comunidades microbianas de água e sedimentos.

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Hydraulic FracturingStreamsMicrobialMolecular SignaturesSediment SamplesNucleic Acid Extraction16S DNA AnalysisMetatranscriptomics RNA AnalysisFilter CollectionPolyethersulfone FilterDNA/RNA Preservative

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Cite this Article

Chen See, J. R., Wright, O., Unverdorben, L. V., Heibeck, N., Techtmann, S. M., Hazen, T. C., Lamendella, R. Evaluating the Impact of Hydraulic Fracturing on Streams using Microbial Molecular Signatures. J. Vis. Exp. (170), e61904, doi:10.3791/61904 (2021).

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