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Laservrije hydroxyl radicale eiwitvoetafdruk om structurele analyse van eiwitten in hogere orde uit te voeren
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Biochemistry
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JoVE Journal Biochemistry
Laser-free Hydroxyl Radical Protein Footprinting to Perform Higher Order Structural Analysis of Proteins
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Laservrije hydroxyl radicale eiwitvoetafdruk om structurele analyse van eiwitten in hogere orde uit te voeren

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3,454 Views

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09:59 min

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June 04, 2021

DOI:

10.3791/61861-v

DOI:
10.3791/61861-v

•

09:59 min
June 04, 2021

•

2 Views
, , ,
1GenNext Technologies Inc., 2Department of Biomolecular Sciences, School of Pharmacy,University of Mississippi, 3Department of Chemistry and Biochemistry,University of Mississippi

Summary

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Dit protocol presenteert een methode om inline radicale dosimetrie en een plasma lichtbron te gebruiken om flash oxidatie eiwit footprinting uit te voeren. Deze methode vervangt de gevaarlijke UV-laser om de reproduceerbaarheid van snelle fotochemische oxidatie van eiwitstudies te vereenvoudigen en te verbeteren.

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Cite this Article

Weinberger, S. R., Chea, E. E., Sharp, J. S., Misra, S. K. Laser-free Hydroxyl Radical Protein Footprinting to Perform Higher Order Structural Analysis of Proteins. J. Vis. Exp. (172), e61861, doi:10.3791/61861 (2021).

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