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Laser-free Hydroxyl Radical Protein Footprinting to Perform Higher Order Structural Analysis of Proteins
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Bioquímica
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JoVE Journal Bioquímica
Laser-free Hydroxyl Radical Protein Footprinting to Perform Higher Order Structural Analysis of Proteins

Laser-free Hydroxyl Radical Protein Footprinting to Perform Higher Order Structural Analysis of Proteins

3,453 Views

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09:59 min

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June 04, 2021

DOI:

10.3791/61861-v

DOI:
10.3791/61861-v

•

09:59 min
June 04, 2021

•

2 Views
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1GenNext Technologies Inc., 2Department of Biomolecular Sciences, School of Pharmacy,University of Mississippi, 3Department of Chemistry and Biochemistry,University of Mississippi

Summary

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This protocol presents a method to use inline radical dosimetry and a plasma light source to perform flash oxidation protein footprinting. This method replaces the hazardous UV laser to simplify and improve the reproducibility of fast photochemical oxidation of protein studies.

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Cite this Article

Weinberger, S. R., Chea, E. E., Sharp, J. S., Misra, S. K. Laser-free Hydroxyl Radical Protein Footprinting to Perform Higher Order Structural Analysis of Proteins. J. Vis. Exp. (172), e61861, doi:10.3791/61861 (2021).

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