Journal
/
生物化学
/
Laser-free Hydroxyl Radical Protein Footprinting to Perform Higher Order Structural Analysis of Proteins
JoVE 杂志
生物化学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物化学
Laser-free Hydroxyl Radical Protein Footprinting to Perform Higher Order Structural Analysis of Proteins

Laser-free Hydroxyl Radical Protein Footprinting to Perform Higher Order Structural Analysis of Proteins

3,455 Views

•

09:59 min

•

June 04, 2021

DOI:

10.3791/61861-v

DOI:
10.3791/61861-v

•

09:59 min
June 04, 2021

•

2 Views
, , ,
1GenNext Technologies Inc., 2Department of Biomolecular Sciences, School of Pharmacy,University of Mississippi, 3Department of Chemistry and Biochemistry,University of Mississippi

Summary

Automatically generated

This protocol presents a method to use inline radical dosimetry and a plasma light source to perform flash oxidation protein footprinting. This method replaces the hazardous UV laser to simplify and improve the reproducibility of fast photochemical oxidation of protein studies.

Explore More Videos

Protein FootprintingHydroxyl RadicalLaser-freeProtein StructureProtein InteractionProtein AggregationProtein StabilityCapillaryPhotolysisDosimetrySilicaPolyimideNutFerrulePhotolysis CellDosimeter CellMagnetic MaskTeflon TubingInjection Loop

Read Article

Cite this Article

Weinberger, S. R., Chea, E. E., Sharp, J. S., Misra, S. K. Laser-free Hydroxyl Radical Protein Footprinting to Perform Higher Order Structural Analysis of Proteins. J. Vis. Exp. (172), e61861, doi:10.3791/61861 (2021).

Download .ris file

Copy

Share Video

.

Copy