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Spotvariations-Fluoreszenz-Korrelationsspektroskopie zur Analyse der molekularen Diffusion an der Plasmamembran lebender Zellen
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
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Spotvariations-Fluoreszenz-Korrelationsspektroskopie zur Analyse der molekularen Diffusion an der Plasmamembran lebender Zellen

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2,441 Views

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05:56 min

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November 12, 2020

DOI:

10.3791/61823-v

DOI:
10.3791/61823-v

•

05:56 min
November 12, 2020

•

4 Views
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1CNRS, Inserm, Centre d’Immunologie de Marseille-Luminy,Aix Marseille Univ, 2Faculty of Biotechnology,University of Wroclaw, 3Faculty of Biological Sciences,University of Wroclaw, 4CNRS, Centrale Marseille, Institut Fresnel,Aix Marseille Univ

Summary

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Dieser Artikel zielt darauf ab, ein Protokoll zum Aufbau eines Fluoreszenz-Korrelationsspektroskopie-Mikroskops (svFCS) zur Messung der molekularen Diffusion an der Plasmamembran lebender Zellen vorzustellen.

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Cite this Article

Mailfert, S., Wojtowicz, K., Brustlein, S., Blaszczak, E., Bertaux, N., Łukaszewicz, M., Marguet, D., Trombik, T. Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells. J. Vis. Exp. (165), e61823, doi:10.3791/61823 (2020).

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