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Journal
/
Biochemistry
/
点变异荧光相关光谱用于分析活细胞质膜上的分子扩散
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
Please note that all translations are automatically generated.
Click here for the English version.
点变异荧光相关光谱用于分析活细胞质膜上的分子扩散
DOI:
10.3791/61823-v
•
05:56 min
•
November 12, 2020
•
Sebastien Mailfert*
1
,
Karolina Wojtowicz*
2
,
Sophie Brustlein*
1
,
Ewa Blaszczak
,
Nicolas Bertaux
,
Marcin Łukaszewicz
,
Didier Marguet
,
Tomasz Trombik
1
CNRS, Inserm, Centre d’Immunologie de Marseille-Luminy
,
Aix Marseille Univ
,
2
Faculty of Biotechnology
,
University of Wroclaw
,
3
Faculty of Biological Sciences
,
University of Wroclaw
,
4
CNRS, Centrale Marseille, Institut Fresnel
,
Aix Marseille Univ
Chapters
00:05
Introduction
00:36
Daily Checkpoint
01:31
Spot Size Calibration
02:36
svFCS Data Acquisition
03:56
Results: Representative svFCS Diffusion Law and Total Cholesterol Comparison
05:07
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文旨在提出一种关于如何构建点变异荧光相关光谱(svFCS)显微镜以测量活细胞质膜处分子扩散的协议。
Tags
Spot Variation Fluorescence Correlation Spectroscopy
Molecular Diffusion
Plasma Membrane
Living Cells
Confocal Microscopy
Laser Power
Rhodamine 6G
Auto Correlation Function
Diffusion Time
SvFCS Analysis
XY Scan
Z Scan
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