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Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

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2,435 Views

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05:56 min

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November 12, 2020

DOI:

10.3791/61823-v

DOI:
10.3791/61823-v

•

05:56 min
November 12, 2020

•

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1CNRS, Inserm, Centre d’Immunologie de Marseille-Luminy,Aix Marseille Univ, 2Faculty of Biotechnology,University of Wroclaw, 3Faculty of Biological Sciences,University of Wroclaw, 4CNRS, Centrale Marseille, Institut Fresnel,Aix Marseille Univ

Summary

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This article aims to present a protocol on how to build a spot variation Fluorescence Correlation Spectroscopy (svFCS) microscope to measure molecular diffusion at the plasma membrane of living cells.

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Cite this Article

Mailfert, S., Wojtowicz, K., Brustlein, S., Blaszczak, E., Bertaux, N., Łukaszewicz, M., Marguet, D., Trombik, T. Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells. J. Vis. Exp. (165), e61823, doi:10.3791/61823 (2020).

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