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質量光測定による抗体-抗原アフィニティーの迅速な測定

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Rapid Determination of Antibody-Antigen Affinity by Mass Photometry

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質量光測定による抗体-抗原アフィニティーの迅速な測定

7,052 Views

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10:17 min

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February 08, 2021

DOI:

10.3791/61784-v

DOI:

10.3791/61784-v

•

10:17 min

February 08, 2021

•

24 Views

Di Wu¹, Grzegorz Piszczek¹

¹Biophysics Core Facility, National Heart, Lung, and Blood Institute,National Institutes of Health

Summary

質量光計測(MP)を用いた抗原抗体親和性測定に対する単一分子アプローチについて述べています。MPベースのプロトコルは、高速で正確で、非常に少量の材料を使用し、タンパク質の改変を必要としません。

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