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Rapid Determination of Antibody-Antigen Affinity by Mass Photometry
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JoVE Journal Biochimica
Rapid Determination of Antibody-Antigen Affinity by Mass Photometry

Rapid Determination of Antibody-Antigen Affinity by Mass Photometry

7,052 Views

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10:17 min

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February 08, 2021

DOI:

10.3791/61784-v

DOI:
10.3791/61784-v

•

10:17 min
February 08, 2021

•

24 Views
,
1Biophysics Core Facility, National Heart, Lung, and Blood Institute,National Institutes of Health

Summary

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We describe a single-molecule approach to antigen-antibody affinity measurements using mass photometry (MP). The MP-based protocol is fast, accurate, uses a very small amount of material, and does not require protein modification.

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Antibody-antigen AffinityMass PhotometryProtein Binding CharacterizationAntibody-antigen BindingProtein OligomerizationProtein-protein InteractionsAffinity MeasurementsRapid Binding AnalysisProtein Sample PreparationFlow Chamber SetupMicroscope Imaging

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Cite this Article

Wu, D., Piszczek, G. Rapid Determination of Antibody-Antigen Affinity by Mass Photometry. J. Vis. Exp. (168), e61784, doi:10.3791/61784 (2021).

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