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Snelle bepaling van antilichaam-antigeen affiniteit door massa fotometrie
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Biochemistry
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JoVE Journal Biochemistry
Rapid Determination of Antibody-Antigen Affinity by Mass Photometry
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Snelle bepaling van antilichaam-antigeen affiniteit door massa fotometrie

Nederlands

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7,052 Views

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10:17 min

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February 08, 2021

DOI:

10.3791/61784-v

DOI:
10.3791/61784-v

•

10:17 min
February 08, 2021

•

24 Views
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1Biophysics Core Facility, National Heart, Lung, and Blood Institute,National Institutes of Health

Summary

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We beschrijven een single-molecule benadering van antigeen-antilichaam affiniteit metingen met behulp van massa fotometrie (MP). Het MP-gebaseerde protocol is snel, nauwkeurig, gebruikt een zeer kleine hoeveelheid materiaal en vereist geen eiwitmodificatie.

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Cite this Article

Wu, D., Piszczek, G. Rapid Determination of Antibody-Antigen Affinity by Mass Photometry. J. Vis. Exp. (168), e61784, doi:10.3791/61784 (2021).

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