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Élimination et remplacement des ligands endogènes des protéines et allergènes liés aux lipides
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Biochemistry
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JoVE Journal Biochemistry
Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens
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Élimination et remplacement des ligands endogènes des protéines et allergènes liés aux lipides

2,823 Views

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09:09 min

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February 24, 2021

DOI:

10.3791/61780-v

DOI:
10.3791/61780-v

•

09:09 min
February 24, 2021

•

4 Views
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1Nuclear Magnetic Resonance Group,National Institute of Environmental Health Sciences

Tags

Endogenous LigandsLipid-bound ProteinsAllergensReverse-phase HPLCThermal AnnealingSolubilizationBla G 1De-lipidationLyophilizationProtein Yield

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Foo, A. C. Y., Thompson, P. M., Mueller, G. A. Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens. J. Vis. Exp. (168), e61780, doi:10.3791/61780 (2021).

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