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Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens
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February 24, 2021
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This protocol describes the removal of endogenous lipids from allergens, and their replacement with user-specified ligands through reverse-phase HPLC coupled with thermal annealing. 31P-NMR and circular dichroism allow for the rapid confirmation of ligand removal/loading, and the recovery of native allergen structure.
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Foo, A. C. Y., Thompson, P. M., Mueller, G. A. Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens. J. Vis. Exp. (168), e61780, doi:10.3791/61780 (2021).
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