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Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens
Kapitel
- 00:04Introduciton
- 01:13Endogenous Lipid Removal
- 02:42Reconstitution of Apo- and Cargo-loaded Bla g 1
- 05:07Phospholipid Cargo Removal/Loading Confirmation
- 06:22Results: Representative Bla g 1 Purification, Lipid Removal Verification, and Recovery
- 08:18Conclusion
This protocol describes the removal of endogenous lipids from allergens, and their replacement with user-specified ligands through reverse-phase HPLC coupled with thermal annealing. 31P-NMR and circular dichroism allow for the rapid confirmation of ligand removal/loading, and the recovery of native allergen structure.
