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Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens

Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens

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09:09 min

February 24, 2021

DOI:

09:09 min
February 24, 2021

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Summary

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This protocol describes the removal of endogenous lipids from allergens, and their replacement with user-specified ligands through reverse-phase HPLC coupled with thermal annealing. 31P-NMR and circular dichroism allow for the rapid confirmation of ligand removal/loading, and the recovery of native allergen structure.

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