Journal
/
/
Quantifying the Effects of Antimicrobials on In vitro Biofilm Architecture using COMSTAT Software
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Quantifying the Effects of Antimicrobials on In vitro Biofilm Architecture using COMSTAT Software

Quantifying the Effects of Antimicrobials on In vitro Biofilm Architecture using COMSTAT Software

2,946 Views

06:18 min

December 14, 2020

DOI:

06:18 min
December 14, 2020

13 Views
, , , ,

Summary

Automatically generated

Antimicrobial-induced changes to Pseudomonas aeruginosa biofilm architecture differ among clinical isolates cultured from patients with cystic fibrosis and chronic pulmonary infection. Following confocal microscopy, COMSTAT software can be utilized to quantify variations in biofilm architecture (e.g., surface area, thickness, biomass) for individual isolates to assess the efficacy of anti-infective agents.

Read Article