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Quantifying the Effects of Antimicrobials on In vitro Biofilm Architecture using COMSTAT Software
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Immunology and Infection
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JoVE Journal Immunology and Infection
Quantifying the Effects of Antimicrobials on In vitro Biofilm Architecture using COMSTAT Software

Quantifying the Effects of Antimicrobials on In vitro Biofilm Architecture using COMSTAT Software

English

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2,946 Views

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06:18 min

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December 14, 2020

DOI:

10.3791/61759-v

DOI:
10.3791/61759-v

•

06:18 min
December 14, 2020

•

13 Views
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1Translational Medicine,Hospital for Sick Children, 2Microbiology, Department of Pediatric Laboratory Medicine,Hospital for Sick Children, 3Infectious Diseases, Department of Pediatrics,Hospital for Sick Children

Summary

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Antimicrobial-induced changes to Pseudomonas aeruginosa biofilm architecture differ among clinical isolates cultured from patients with cystic fibrosis and chronic pulmonary infection. Following confocal microscopy, COMSTAT software can be utilized to quantify variations in biofilm architecture (e.g., surface area, thickness, biomass) for individual isolates to assess the efficacy of anti-infective agents.

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Cite this Article

Morris, A. J., Li, A., Jackson, L., Yau, Y. C. W., Waters, V. Quantifying the Effects of Antimicrobials on In vitro Biofilm Architecture using COMSTAT Software. J. Vis. Exp. (166), e61759, doi:10.3791/61759 (2020).

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