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Quantifying the Effects of Antimicrobials on In vitro Biofilm Architecture using COMSTAT Software
JoVE Journal
免疫学と感染
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JoVE Journal 免疫学と感染
Quantifying the Effects of Antimicrobials on In vitro Biofilm Architecture using COMSTAT Software

Quantifying the Effects of Antimicrobials on In vitro Biofilm Architecture using COMSTAT Software

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06:18 min

December 14, 2020

DOI:

06:18 min
December 14, 2020

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概要

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Antimicrobial-induced changes to Pseudomonas aeruginosa biofilm architecture differ among clinical isolates cultured from patients with cystic fibrosis and chronic pulmonary infection. Following confocal microscopy, COMSTAT software can be utilized to quantify variations in biofilm architecture (e.g., surface area, thickness, biomass) for individual isolates to assess the efficacy of anti-infective agents.

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