/
/
Quantifying the Effects of Antimicrobials on In vitro Biofilm Architecture using COMSTAT Software
Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Quantifying the Effects of Antimicrobials on In vitro Biofilm Architecture using COMSTAT Software
2,946 Views
•
06:18 min
•
December 14, 2020
DOI:
Summary
Automatically generated
Antimicrobial-induced changes to Pseudomonas aeruginosa biofilm architecture differ among clinical isolates cultured from patients with cystic fibrosis and chronic pulmonary infection. Following confocal microscopy, COMSTAT software can be utilized to quantify variations in biofilm architecture (e.g., surface area, thickness, biomass) for individual isolates to assess the efficacy of anti-infective agents.
Read Article
Cite this Article
Morris, A. J., Li, A., Jackson, L., Yau, Y. C. W., Waters, V. Quantifying the Effects of Antimicrobials on In vitro Biofilm Architecture using COMSTAT Software. J. Vis. Exp. (166), e61759, doi:10.3791/61759 (2020).
Copy