Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

Ragini  M. Mistry; Pankaj  K. Singh; Maureen  G. Mancini; Fabio Stossi; Michael  A. Mancini

doi:10.3791/61680

Journal

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Biochemistry

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Transkripsiyonel Aktif Alellerin Tek Molekül FISH ile Tek Hücre Analizi

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Biochemistry

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JoVE Journal Biochemistry

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

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Transkripsiyonel Aktif Alellerin Tek Molekül FISH ile Tek Hücre Analizi

2,990 Views

•

06:26 min

•

September 20, 2020

DOI:

10.3791/61680-v

DOI:

10.3791/61680-v

•

06:26 min

September 20, 2020

•

4 Views

Ragini M. Mistry^1,2, Pankaj K. Singh^1,3, Maureen G. Mancini^1,2, Fabio Stossi^1,2, Michael A. Mancini^1,2,3,4

¹GCC Center for Advanced Microscopy and Image Informatics, ²Department of Molecular and Cellular Biology,Baylor College of Medicine, ³Center for Translational Cancer Research, Institute of Biosciences and Technology,Texas A&M University, ⁴Department of Pharmacology and Chemical Biology,Baylor College of Medicine

Summary

Tek moleküllü RNA floresan in situ hibridizasyon (smFISH), belirli RNA'ların seviyelerini ve lokalizasyonunu tek hücre düzeyinde doğru bir şekilde ölçmek için bir yöntemdir. Burada, belirli RNA'ların tek hücreli nicelleştirilmesi için ıslak tezgah işleme, görüntüleme ve görüntü analizi için onaylanmış laboratuvar protokollerimizi rapor ediyoruz.

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Transkripsiyonel Aktif Alellerin Tek Molekül FISH ile Tek Hücre Analizi

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Summary

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