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単一分子FISHによる転写活性アレスの単一細胞解析

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

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単一分子FISHによる転写活性アレスの単一細胞解析

2,990 Views

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06:26 min

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September 20, 2020

DOI:

10.3791/61680-v

DOI:

10.3791/61680-v

•

06:26 min

September 20, 2020

•

4 Views

Ragini M. Mistry^1,2, Pankaj K. Singh^1,3, Maureen G. Mancini^1,2, Fabio Stossi^1,2, Michael A. Mancini^1,2,3,4

¹GCC Center for Advanced Microscopy and Image Informatics, ²Department of Molecular and Cellular Biology,Baylor College of Medicine, ³Center for Translational Cancer Research, Institute of Biosciences and Technology,Texas A&M University, ⁴Department of Pharmacology and Chemical Biology,Baylor College of Medicine

Summary

この場合の単一分子RNA蛍光蛍光(smFISH)は、単一細胞レベルで特異的なRNAのレベルおよび局在を正確に定量する方法です。ここでは、特定のRNAの単一細胞定量化のためのウェットベンチ処理、イメージングおよび画像解析のための検証済みのラボプロトコルを報告します。

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