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Isolamento e Identificação Conjuntivival Commensal em Camundongos
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07:52 min
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May 01, 2021
DOI:
1Department of Medicine, Division of Infectious Diseases, Brigham and Women’s Hospital,Harvard Medical School, 2Massachusetts Host-Microbiome Center, Department of Pathology, Brigham and Women’s Hospital,Harvard Medical School, 3Clinical Microbiology Laboratory, Department of Pathology, Brigham and Women’s Hospital,Harvard Medical School
Summary
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Apresentado aqui é um protocolo para o isolamento e amplificação de bactérias comensais anaeróbicas aeróbicas e facultativas de camundongos anastrérmicos utilizando um cotonete de olho único e passo de enriquecimento baseado em cultura com identificação subsequente por métodos à base microbiológica e espectrometria de massa MALDI-TOF.
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Smith-Page, K., Kugadas, A., Lin, T., Delaney, M., Bry, L., Gadjeva, M. Conjunctival Commensal Isolation and Identification in Mice. J. Vis. Exp. (171), e61672, doi:10.3791/61672 (2021).
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