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07:52 min
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May 01, 2021
DOI:
1Department of Medicine, Division of Infectious Diseases, Brigham and Women’s Hospital,Harvard Medical School, 2Massachusetts Host-Microbiome Center, Department of Pathology, Brigham and Women’s Hospital,Harvard Medical School, 3Clinical Microbiology Laboratory, Department of Pathology, Brigham and Women’s Hospital,Harvard Medical School
Summary
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ここでは、微生物学的手法とMALDI-TOF質量分析によるその後の同定を用いたユニークな眼綿棒および培養ベースの濃縮ステップを用いた有酸素性および気性嫌気性マウスの結合性のコンメンサルト菌の分離と増幅のためのプロトコルを提示する。
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Cite this Article
Smith-Page, K., Kugadas, A., Lin, T., Delaney, M., Bry, L., Gadjeva, M. Conjunctival Commensal Isolation and Identification in Mice. J. Vis. Exp. (171), e61672, doi:10.3791/61672 (2021).
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