このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
Conjunctival Commensal Isolation and Identification in Mice
4,154 Views
•
07:52 min
•
May 01, 2021
DOI:
1Department of Medicine, Division of Infectious Diseases, Brigham and Women’s Hospital,Harvard Medical School, 2Massachusetts Host-Microbiome Center, Department of Pathology, Brigham and Women’s Hospital,Harvard Medical School, 3Clinical Microbiology Laboratory, Department of Pathology, Brigham and Women’s Hospital,Harvard Medical School
概要
Automatically generated
Presented here is a protocol for the isolation and amplification of aerobic and facultative anaerobic mouse conjunctival commensal bacteria using a unique eye swab and culture-based enrichment step with subsequent identification by microbiological based methods and MALDI-TOF mass spectrometry.
Read Article
Cite this Article
Smith-Page, K., Kugadas, A., Lin, T., Delaney, M., Bry, L., Gadjeva, M. Conjunctival Commensal Isolation and Identification in Mice. J. Vis. Exp. (171), e61672, doi:10.3791/61672 (2021).
Copy