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Einzelzell-Elektroporation über verschiedene organotypische Slice-Kulturen von Hippocampus-Exzitatorischen und klassenspezifischen inhibitorischen Neuronen der Maus
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JoVE Journal Neuroscience
Single-Cell Electroporation across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neurons
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Einzelzell-Elektroporation über verschiedene organotypische Slice-Kulturen von Hippocampus-Exzitatorischen und klassenspezifischen inhibitorischen Neuronen der Maus

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3,384 Views

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06:21 min

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October 06, 2020

DOI:

10.3791/61662-v

DOI:
10.3791/61662-v

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06:21 min
October 06, 2020

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3 Views
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1Brudnick Neuropsychiatric Research Institute, Department of Neurobiology,University of Massachusetts Medical School, 2Interdisciplinary Graduate Program,University of Massachusetts Medical School, 3UMMS MD/PhD Program,University of Massachusetts Medical School

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Single-cell ElectroporationOrganotypic Slice CultureMouse HippocampusExcitatory NeuronsInhibitory NeuronsGene TransfectionNeurobiologyElectroporation ProtocolHigh Transfection EfficiencyCell-autonomous MechanismsTranssynaptic Protein InteractionsSlice Culture PreparationElectroporator SetupPipette PreparationSlice Culture IsolationTarget Cell Electroporation

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Keener, D. G., Cheung, A., Futai, K. Single-Cell Electroporation across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neurons. J. Vis. Exp. (164), e61662, doi:10.3791/61662 (2020).

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