/
/
Single-Cell Electroporation across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neurons
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Single-Cell Electroporation across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neurons
3,403 Views
•
06:21 min
•
October 06, 2020
DOI:
Summary
Automatically generated
Presented here is a protocol for single-cell electroporation that can deliver genes in both excitatory and inhibitory neurons across a range of in vitro hippocampal slice culture ages. Our approach provides precise and efficient expression of genes in individual cells, which can be used to examine cell-autonomous and intercellular functions.
Explore More Videos
Single-cell ElectroporationOrganotypic Slice CultureMouse HippocampusExcitatory NeuronsInhibitory NeuronsGene TransfectionNeurobiologyElectroporation ProtocolHigh Transfection EfficiencyCell-autonomous MechanismsTranssynaptic Protein InteractionsSlice Culture PreparationElectroporator SetupPipette PreparationSlice Culture IsolationTarget Cell Electroporation
Read Article
Cite this Article
Keener, D. G., Cheung, A., Futai, K. Single-Cell Electroporation across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neurons. J. Vis. Exp. (164), e61662, doi:10.3791/61662 (2020).
Copy