Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Single-Cell Electroporation across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neurons
3,398 Views
•
06:21 min
•
October 06, 2020
DOI:
Özet
Automatically generated
Presented here is a protocol for single-cell electroporation that can deliver genes in both excitatory and inhibitory neurons across a range of in vitro hippocampal slice culture ages. Our approach provides precise and efficient expression of genes in individual cells, which can be used to examine cell-autonomous and intercellular functions.
Explore More Videos
Single-cell ElectroporationOrganotypic Slice CultureMouse HippocampusExcitatory NeuronsInhibitory NeuronsGene TransfectionNeurobiologyElectroporation ProtocolHigh Transfection EfficiencyCell-autonomous MechanismsTranssynaptic Protein InteractionsSlice Culture PreparationElectroporator SetupPipette PreparationSlice Culture IsolationTarget Cell Electroporation
Read Article
Cite this Article
Keener, D. G., Cheung, A., Futai, K. Single-Cell Electroporation across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neurons. J. Vis. Exp. (164), e61662, doi:10.3791/61662 (2020).
Copy