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重建线粒体膜动力学的模型膜平台
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal Biochemistry
A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics
DOI:

10:31 min

September 02, 2020

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Chapters

  • 00:04Introduction
  • 00:37Fabrication of Lipid Bilayers
  • 04:41Protein Reconstitution into the Polymer-tethered Lipid Bilayer
  • 06:02Preparation of Proteoliposomes
  • 07:46Results: Reconstituted Transmembrane Protein Diffusion and Detection of Membrane Tethering, Lipid Hemifusion, and Pore Opening
  • 09:42Conclusion

Summary

Automatic Translation

线粒体融合是线粒体动力学基础的重要恒态反应。这里描述的是 一个体外 重组系统,研究线粒体内膜融合,可以解决膜系绳、对接、血融液和孔隙开口。讨论了该方法在探索细胞膜系统的多功能性。

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