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Biochemistry
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重建线粒体膜动力学的模型膜平台
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics
Please note that all translations are automatically generated.
Click here for the English version.
重建线粒体膜动力学的模型膜平台
DOI:
10.3791/61620-v
•
10:31 min
•
September 02, 2020
•
Yifan Ge
,
Sivakumar Boopathy
,
Adam Smith
,
Luke H. Chao
3
1
Department of Molecular Biology
,
Massachusetts General Hospital
,
2
Department of Chemistry
,
University of Akron
,
3
Department of Genetics
,
Harvard Medical School
Chapters
00:04
Introduction
00:37
Fabrication of Lipid Bilayers
04:41
Protein Reconstitution into the Polymer-tethered Lipid Bilayer
06:02
Preparation of Proteoliposomes
07:46
Results: Reconstituted Transmembrane Protein Diffusion and Detection of Membrane Tethering, Lipid Hemifusion, and Pore Opening
09:42
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
线粒体融合是线粒体动力学基础的重要恒态反应。这里描述的是
一个体外
重组系统,研究线粒体内膜融合,可以解决膜系绳、对接、血融液和孔隙开口。讨论了该方法在探索细胞膜系统的多功能性。
Tags
Mitochondria
Membrane Dynamics
In Vitro Reconstitution
Lipid Environment
Inner Membrane Fusion
Integral Membrane Proteins
Membrane-associated Proteins
Langmuir-Blodgett
Lipid Bilayer
Chloroform
Sodium Hydroxide
Methanol
Hydrochloric Acid
Polytetrafluoroethylene
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