Journal
/
Bioquímica
/
A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics
JoVE Revista
Bioquímica
This content is Free Access.
JoVE Revista Bioquímica
A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics

A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics

7,177 Views

•

10:31 min

•

September 02, 2020

DOI:

10.3791/61620-v

DOI:
10.3791/61620-v

•

10:31 min
September 02, 2020

•

17 Views
, , ,
1Department of Molecular Biology,Massachusetts General Hospital, 2Department of Chemistry,University of Akron, 3Department of Genetics,Harvard Medical School

Summary

Automatically generated

Mitochondrial fusion is an important homeostatic reaction underlying mitochondrial dynamics. Described here is an in vitro reconstitution system to study mitochondrial inner-membrane fusion that can resolve membrane tethering, docking, hemifusion, and pore opening. The versatility of this approach in exploring cell membrane systems is discussed.

Explore More Videos

MitochondriaMembrane DynamicsIn Vitro ReconstitutionLipid EnvironmentInner Membrane FusionIntegral Membrane ProteinsMembrane-associated ProteinsLangmuir-BlodgettLipid BilayerChloroformSodium HydroxideMethanolHydrochloric AcidPolytetrafluoroethylene

Read Article

Cite this Article

Ge, Y., Boopathy, S., Smith, A., Chao, L. H. A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics. J. Vis. Exp. (163), e61620, doi:10.3791/61620 (2020).

Download .ris file

Copy

Share Video

.

Copy