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A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics

A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics

DOI:
10.3791/61620-v

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10:31 min

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September 02, 2020

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, , ,
1Department of Molecular Biology,Massachusetts General Hospital, 2Department of Chemistry,University of Akron, 3Department of Genetics,Harvard Medical School

Chapters

  • 00:04Introduction
  • 00:37Fabrication of Lipid Bilayers
  • 04:41Protein Reconstitution into the Polymer-tethered Lipid Bilayer
  • 06:02Preparation of Proteoliposomes
  • 07:46Results: Reconstituted Transmembrane Protein Diffusion and Detection of Membrane Tethering, Lipid Hemifusion, and Pore Opening
  • 09:42Conclusion

Summary

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Mitochondrial fusion is an important homeostatic reaction underlying mitochondrial dynamics. Described here is an in vitro reconstitution system to study mitochondrial inner-membrane fusion that can resolve membrane tethering, docking, hemifusion, and pore opening. The versatility of this approach in exploring cell membrane systems is discussed.

Tags

MitochondriaMembrane DynamicsIn Vitro ReconstitutionLipid EnvironmentInner Membrane FusionIntegral Membrane ProteinsMembrane-associated ProteinsLangmuir-BlodgettLipid BilayerChloroformSodium HydroxideMethanolHydrochloric AcidPolytetrafluoroethylene

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