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A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics

A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics

7,177 Views

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10:31 min

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September 02, 2020

DOI:

10.3791/61620-v

DOI:
10.3791/61620-v

•

10:31 min
September 02, 2020

•

17 Views
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1Department of Molecular Biology,Massachusetts General Hospital, 2Department of Chemistry,University of Akron, 3Department of Genetics,Harvard Medical School

Summary

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Mitochondrial fusion is an important homeostatic reaction underlying mitochondrial dynamics. Described here is an in vitro reconstitution system to study mitochondrial inner-membrane fusion that can resolve membrane tethering, docking, hemifusion, and pore opening. The versatility of this approach in exploring cell membrane systems is discussed.

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Cite this Article

Ge, Y., Boopathy, S., Smith, A., Chao, L. H. A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics. J. Vis. Exp. (163), e61620, doi:10.3791/61620 (2020).

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