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Sondare la cinetica dell'mRNA nello spazio e nel tempo in Escherichia coli utilizzando la fluorescenza a singola molecola a due colori in ibridazione situ
JoVE Journal
Biology
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JoVE Journal Biology
Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization
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Sondare la cinetica dell'mRNA nello spazio e nel tempo in Escherichia coli utilizzando la fluorescenza a singola molecola a due colori in ibridazione situ

italiano

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6,763 Views

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10:01 min

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July 30, 2020

DOI:

10.3791/61520-v

DOI:
10.3791/61520-v

•

10:01 min
July 30, 2020

•

11 Views
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1Department of Physics,University of Illinois at Urbana-Champaign, 2Center for the Physics of Living Cells,University of Illinois at Urbana-Champaign, 3Carl R. Woese Institute for Genomic Biology,University of Illinois at Urbana-Champaign, 4Center for Biophysics and Quantitative Biology,University of Illinois at Urbana-Champaign

Summary

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Questo protocollo descrive un'applicazione di fluorescenza a singola molecola in ibridazione situ (smFISH) per misurare la cinetica in vivo della sintesi e della degradazione dell'mRNA.

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Kim, S., Vaidya, K. Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization. J. Vis. Exp. (161), e61520, doi:10.3791/61520 (2020).

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