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Sondando cinética mRNA no espaço e no tempo em Escherichia coli usando fluorescência de molécula única de duas cores na hibridização situ
JoVE Journal
Biology
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JoVE Journal Biology
Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization

Sondando cinética mRNA no espaço e no tempo em Escherichia coli usando fluorescência de molécula única de duas cores na hibridização situ

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6,754 Views

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10:01 min

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July 30, 2020

DOI:

10.3791/61520-v

DOI:
10.3791/61520-v

•

10:01 min
July 30, 2020

•

11 Views
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1Department of Physics,University of Illinois at Urbana-Champaign, 2Center for the Physics of Living Cells,University of Illinois at Urbana-Champaign, 3Carl R. Woese Institute for Genomic Biology,University of Illinois at Urbana-Champaign, 4Center for Biophysics and Quantitative Biology,University of Illinois at Urbana-Champaign

Summary

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Este protocolo descreve uma aplicação de fluorescência de molécula única na hibridização situ (smFISH) para medir a cinética in vivo da síntese e degradação do mRNA.

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Kim, S., Vaidya, K. Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization. J. Vis. Exp. (161), e61520, doi:10.3791/61520 (2020).

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