Journal
/
Biology
/
Situ Hibridizasyonda İki Renkli Tek Moleküllü Floresan Kullanarak Escherichia coli'de Uzay ve Zamanda MRNA KinetikLerini Sondalama
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization
Please note that all translations are automatically generated. Click here for the English version.

Situ Hibridizasyonda İki Renkli Tek Moleküllü Floresan Kullanarak Escherichia coli'de Uzay ve Zamanda MRNA KinetikLerini Sondalama

Türkçe

Automatically Generated

6,754 Views

•

10:01 min

•

July 30, 2020

DOI:

10.3791/61520-v

DOI:
10.3791/61520-v

•

10:01 min
July 30, 2020

•

11 Views
,
1Department of Physics,University of Illinois at Urbana-Champaign, 2Center for the Physics of Living Cells,University of Illinois at Urbana-Champaign, 3Carl R. Woese Institute for Genomic Biology,University of Illinois at Urbana-Champaign, 4Center for Biophysics and Quantitative Biology,University of Illinois at Urbana-Champaign

Summary

Automatically generated

Bu protokol, mRNA sentezi ve bozulmasının in vivo kinetiklerini ölçmek için yerinde hibridizasyonda (smFISH) tek moleküllü floresan uygulamasını açıklar.

Explore More Videos

MRNA KineticsTwo-color Single-molecule Fluorescence In Situ Hybridization (smFISH)TranscriptionMRNA DegradationEscherichia ColiTime Course ExperimentIPTG InductionONPF RepressionCell FixationCell Permeabilization

Read Article

Cite this Article

Kim, S., Vaidya, K. Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization. J. Vis. Exp. (161), e61520, doi:10.3791/61520 (2020).

Download .ris file

Copy

Share Video

.

Copy