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Aislamiento de miofiber único y cultura de un modelo murino de distrofia muscular de Emery-Dreifuss en el desarrollo post-Natal temprano
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Medicine
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JoVE Journal Medicine
Single Myofiber Isolation and Culture from a Murine Model of Emery-Dreifuss Muscular Dystrophy in Early Post-Natal Development
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Aislamiento de miofiber único y cultura de un modelo murino de distrofia muscular de Emery-Dreifuss en el desarrollo post-Natal temprano

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7,231 Views

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08:07 min

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July 01, 2020

DOI:

10.3791/61516-v

DOI:
10.3791/61516-v

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08:07 min
July 01, 2020

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5 Views
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1Istituto Nazionale di Genetica Molecolare “Romeo ed Enrica Invernizzi”, 2IRCCS Santa Lucia Foundation, 3Department of Biology and Biotechnology, “C. Darwin” Sapienza University,CNR – IBPM, 4CNR – Institute of Biomedical Technologies (ITB)

Summary

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Aquí, proponemos un método para obtener eficientemente fibras musculares individuales en etapas de desarrollo postnatal tempranas a partir del modelo de ratón lamin 8-11 mutante homocigoto, un modelo muy severo para la distrofia muscular de Emery-Dreifuss (EDMD).

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Cite this Article

Pegoli, G., Lucini, F., Mozzetta, C., Lanzuolo, C. Single Myofiber Isolation and Culture from a Murine Model of Emery-Dreifuss Muscular Dystrophy in Early Post-Natal Development. J. Vis. Exp. (161), e61516, doi:10.3791/61516 (2020).

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