Journal
/
Medizin
/
Single Myofiber Isolation and Culture from a Murine Model of Emery-Dreifuss Muscular Dystrophy in Early Post-Natal Development
JoVE Journal
Medizin
This content is Free Access.
JoVE Journal Medizin
Single Myofiber Isolation and Culture from a Murine Model of Emery-Dreifuss Muscular Dystrophy in Early Post-Natal Development

Single Myofiber Isolation and Culture from a Murine Model of Emery-Dreifuss Muscular Dystrophy in Early Post-Natal Development

7,233 Views

•

08:07 min

•

July 01, 2020

DOI:

10.3791/61516-v

DOI:
10.3791/61516-v

•

08:07 min
July 01, 2020

•

5 Views
, , ,
1Istituto Nazionale di Genetica Molecolare “Romeo ed Enrica Invernizzi”, 2IRCCS Santa Lucia Foundation, 3Department of Biology and Biotechnology, “C. Darwin” Sapienza University,CNR – IBPM, 4CNR – Institute of Biomedical Technologies (ITB)

Summary

Automatically generated

Here, we propose a method to efficiently obtain single muscle fibers at early post-natal developmental stages from homozygous mutant Lamin Δ8-11 mouse model, a very severe model for Emery-Dreifuss muscular dystrophy (EDMD).

Explore More Videos

Single Myofiber IsolationEmery-Dreifuss Muscular DystrophyPostnatal DevelopmentMuscle Stem CellsMuscle DissectionMuscle DigestionMuscle Fiber IsolationWashing SolutionPetri Dish

Read Article

Cite this Article

Pegoli, G., Lucini, F., Mozzetta, C., Lanzuolo, C. Single Myofiber Isolation and Culture from a Murine Model of Emery-Dreifuss Muscular Dystrophy in Early Post-Natal Development. J. Vis. Exp. (161), e61516, doi:10.3791/61516 (2020).

Download .ris file

Copy

Share Video

.

Copy