Here, we propose a method to efficiently obtain single muscle fibers at early post-natal developmental stages from homozygous mutant Lamin Δ8-11 mouse model, a very severe model for Emery-Dreifuss muscular dystrophy (EDMD).
Pegoli, G., Lucini, F., Mozzetta, C., Lanzuolo, C. Single Myofiber Isolation and Culture from a Murine Model of Emery-Dreifuss Muscular Dystrophy in Early Post-Natal Development. J. Vis. Exp. (161), e61516, doi:10.3791/61516 (2020).