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Single Myofiber Isolation and Culture from a Murine Model of Emery-Dreifuss Muscular Dystrophy in Early Post-Natal Development
JoVE 杂志
医学
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JoVE 杂志 医学
Single Myofiber Isolation and Culture from a Murine Model of Emery-Dreifuss Muscular Dystrophy in Early Post-Natal Development

Single Myofiber Isolation and Culture from a Murine Model of Emery-Dreifuss Muscular Dystrophy in Early Post-Natal Development

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08:07 min

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July 01, 2020

DOI:

10.3791/61516-v

DOI:
10.3791/61516-v

•

08:07 min
July 01, 2020

•

5 Views
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1Istituto Nazionale di Genetica Molecolare “Romeo ed Enrica Invernizzi”, 2IRCCS Santa Lucia Foundation, 3Department of Biology and Biotechnology, “C. Darwin” Sapienza University,CNR – IBPM, 4CNR – Institute of Biomedical Technologies (ITB)

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Here, we propose a method to efficiently obtain single muscle fibers at early post-natal developmental stages from homozygous mutant Lamin Δ8-11 mouse model, a very severe model for Emery-Dreifuss muscular dystrophy (EDMD).

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Pegoli, G., Lucini, F., Mozzetta, C., Lanzuolo, C. Single Myofiber Isolation and Culture from a Murine Model of Emery-Dreifuss Muscular Dystrophy in Early Post-Natal Development. J. Vis. Exp. (161), e61516, doi:10.3791/61516 (2020).

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