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SAX-HPLC를 사용하여 다른 식물 종에서 용해성, 방사성 라벨이 붙은 이노시톨 폴리인산염의 추출 및 정량화
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal Biochemistry
Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
DOI:

09:01 min

June 26, 2020

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Chapters

  • 00:04Introduction
  • 00:50HPLC System Set Up
  • 01:37Plant Cultivation and Labeling with [3H]-myo-inositol
  • 03:02Extraction of Soluble InsPs
  • 04:31HPLC
  • 05:51Data Analysis
  • 06:45Results: InsP profiles of A. thaliana and L. japonicus
  • 08:15Conclusion

Summary

Automatic Translation

여기서는식물에서이노시톨 폴리인산염을 검출하고 정량화하는 매우 민감한 방법입니다.myo

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