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SAX-HPLCを用いた異種植物由来の可溶性、放射性標識イノシトールポリリン酸塩の抽出と定量化
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
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SAX-HPLCを用いた異種植物由来の可溶性、放射性標識イノシトールポリリン酸塩の抽出と定量化

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6,615 Views

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09:01 min

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June 26, 2020

DOI:

10.3791/61495-v

DOI:
10.3791/61495-v

•

09:01 min
June 26, 2020

•

11 Views
, , ,
1Department of Plant Nutrition, Institute of Crop Science and Resource Conservation,University of Bonn

Summary

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ここでは、植物中のイノシトールポリリン酸塩を検出し定量する高感度の方法である[3H]-ミオ-イノシトール標識苗の強力なアニオン交換高速液体クロマトグラフィーについて述べた。

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Cite this Article

Gaugler, P., Gaugler, V., Kamleitner, M., Schaaf, G. Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC. J. Vis. Exp. (160), e61495, doi:10.3791/61495 (2020).

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