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使用SAX-HPLC从不同植物物种中提取和定量可溶性、放射性标记肌醇多磷酸盐
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
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使用SAX-HPLC从不同植物物种中提取和定量可溶性、放射性标记肌醇多磷酸盐

English

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6,562 Views

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09:01 min

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June 26, 2020

DOI:

10.3791/61495-v

DOI:
10.3791/61495-v

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09:01 min
June 26, 2020

•

11 Views
, , ,
1Department of Plant Nutrition, Institute of Crop Science and Resource Conservation,University of Bonn

Summary

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在这里，我们描述了强抗离子交换高性能液相色谱的[3H]-肌醇标记幼苗，这是一种高度敏感的方法，用于检测和量化植物中的肌醇聚磷酸盐。

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Gaugler, P., Gaugler, V., Kamleitner, M., Schaaf, G. Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC. J. Vis. Exp. (160), e61495, doi:10.3791/61495 (2020).

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