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Journal
/
Biochemistry
/
分析阿拉伯
恐惧症中渗透应激信号的磷蛋白分析策略
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in
Arabidopsis
Please note that all translations are automatically generated.
Click here for the English version.
分析阿拉伯
恐惧症中渗透应激信号的磷蛋白分析策略
DOI:
10.3791/61489-v
•
05:47 min
•
June 25, 2020
•
Chuan-Chih Hsu
3
,
Chia-Feng Tsai
,
W. Andy Tao
4
,
Pengcheng Wang
1
Department of Plant Biology
,
Carnegie Institute for Science
,
2
Graduate School of Pharmaceutical Sciences
,
Kyoto University
,
3
Department of Biochemistry
,
Purdue University
,
4
Department of Chemistry
,
Purdue University
,
5
Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences
,
Chinese Academy of Sciences
Chapters
00:04
Introduction
00:33
Immobilized Metal Ion Affinity Chromatography (IMAC) and High pH Reversed-Phase (Hp-RP) Stage Tip Preparation
01:10
IMAC Stage Tip Phosphopeptide Enrichment
02:44
Hp-RP C18 Stage Tip Phosphopeptide Fractionation
03:37
Results: Representative Osmotic Stress Signaling in Arabidopsis
05:05
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里介绍的是一种磷蛋白法,即停止和去提取基于磷蛋白的尖端,它提供了高通量和深度覆盖
的阿拉比多普西
磷蛋白。这种方法概述了阿拉伯语中渗透应激信号
的概述。
Tags
Phosphoproteomics
Osmotic Stress
Arabidopsis
IMAC
Phosphopeptide Enrichment
High PH Reverse Phase Fractionation
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