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Extra Cellular Matrix-Based und Extra Cellular Matrix-Free Generation of Murine Hodenorganoide
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Developmental Biology
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JoVE Journal Developmental Biology
Extra Cellular Matrix-Based and Extra Cellular Matrix-Free Generation of Murine Testicular Organoids
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Extra Cellular Matrix-Based und Extra Cellular Matrix-Free Generation of Murine Hodenorganoide

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6,596 Views

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09:18 min

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October 07, 2020

DOI:

10.3791/61403-v

DOI:
10.3791/61403-v

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09:18 min
October 07, 2020

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8 Views
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1Department of Obstetrics and Gynecology, Feinberg School of Medicine,Northwestern University

Summary

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Hier werden vier Methoden zur Erzeugung von Hodenorganoiden aus primären neonatalen murinen Hodenzellzellen beschrieben, d.h. extrazelluläre Matrix (ECM) und ECM-freie 2D- und 3D-Kulturumgebungen. Diese Techniken haben mehrere Forschungsanwendungen und sind besonders nützlich für das Studium der Hodenentwicklung und Physiologie in vitro.

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Edmonds, M. E., Forshee, M. D., Woodruff, T. K. Extra Cellular Matrix-Based and Extra Cellular Matrix-Free Generation of Murine Testicular Organoids. J. Vis. Exp. (164), e61403, doi:10.3791/61403 (2020).

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