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JoVE Journal Developmental Biology
Extra Cellular Matrix-Based and Extra Cellular Matrix-Free Generation of Murine Testicular Organoids
 

Extra Cellular Matrix-Based and Extra Cellular Matrix-Free Generation of Murine Testicular Organoids

Article DOI: 10.3791/61403-v • 09:18 min • October 7th, 2020
October 7th, 2020 •
1, 1, 1
1Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University

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Here, four methods for generating testicular organoids from primary neonatal murine testicular cells are described i.e., extracellular matrix (ECM) and ECM-free 2D and 3D culture environments. These techniques have multiple research applications and are especially useful for studying testicular development and physiology in vitro.

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Extracellular Matrix-based Extracellular Matrix-free Testicular Organoids 2D Culture 3D Culture Structurally Mimetic Endocrine Functional Reproducible Techniques Standard Biological Laboratory Settings Common Tools And Materials Simulating Spermatogenesis Reproductive Endocrinology In Vitro Gametogenesis Euthanized Mouse Dissection Mat Sterilizing Abdomen Testes Vas Deferens Anchoring Connective Tissue Tunica Albuginea Micro Dissection Scissors Fine Forceps
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