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Extra Cellular Matrix-Based and Extra Cellular Matrix-Free Generation of Murine Testicular Organoids
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October 07, 2020
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Here, four methods for generating testicular organoids from primary neonatal murine testicular cells are described i.e., extracellular matrix (ECM) and ECM-free 2D and 3D culture environments. These techniques have multiple research applications and are especially useful for studying testicular development and physiology in vitro.
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Edmonds, M. E., Forshee, M. D., Woodruff, T. K. Extra Cellular Matrix-Based and Extra Cellular Matrix-Free Generation of Murine Testicular Organoids. J. Vis. Exp. (164), e61403, doi:10.3791/61403 (2020).
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