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Delivery of Antibodies into the Brain Using Focused Scanning Ultrasound
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Neuroscience
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JoVE Journal Neuroscience
Delivery of Antibodies into the Brain Using Focused Scanning Ultrasound

Delivery of Antibodies into the Brain Using Focused Scanning Ultrasound

English

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2,791 Views

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07:34 min

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July 18, 2020

DOI:

10.3791/61372-v

DOI:
10.3791/61372-v

•

07:34 min
July 18, 2020

•

5 Views
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1Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute,The University of Queensland

Summary

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Presented here is a protocol to transiently open the blood-brain barrier (BBB) either focally or throughout a mouse brain to deliver fluorescently-labeled antibodies and activate microglia. Also presented is a method to detect the delivery of antibodies and microglia activation by histology.

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Cite this Article

Leinenga, G., Bodea, L., Koh, W. K., Nisbet, R. M., Götz, J. Delivery of Antibodies into the Brain Using Focused Scanning Ultrasound. J. Vis. Exp. (161), e61372, doi:10.3791/61372 (2020).

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