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Delivery of Antibodies into the Brain Using Focused Scanning Ultrasound
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Neurociência
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JoVE Journal Neurociência
Delivery of Antibodies into the Brain Using Focused Scanning Ultrasound

Delivery of Antibodies into the Brain Using Focused Scanning Ultrasound

DOI:
10.3791/61372-v

•

07:34 min

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July 18, 2020

•

, , , ,
1Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute,The University of Queensland

Capítulos

  • 00:04Introduction
  • 00:34Microbubble Quality Control
  • 02:11Fluorescent Antibody Labeling
  • 02:46Focused Scanning Ultrasound Setup (SUS)
  • 04:29Ultrasound Preparation
  • 04:52SUS Treatment
  • 06:12Results: Representative SUS Antibody Delivery Evaluation
  • 06:48Conclusion

Summary

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Presented here is a protocol to transiently open the blood-brain barrier (BBB) either focally or throughout a mouse brain to deliver fluorescently-labeled antibodies and activate microglia. Also presented is a method to detect the delivery of antibodies and microglia activation by histology.

Tags

AntibodiesBlood-brain BarrierFocused Scanning UltrasoundMicrobubblesCell CounterFluorescent LabelingMouse IgG AntibodyProtein ConcentrationAnnular Array

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