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切除小鼠神经神经纤维神经丝的成像与分析
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Neuroscience
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JoVE Journal Neuroscience
Imaging and Analysis of Neurofilament Transport in Excised Mouse Tibial Nerve
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切除小鼠神经神经纤维神经丝的成像与分析

DOI:
10.3791/61264-v

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09:52 min

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August 31, 2020

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, , ,
1Department of Neuroscience,The Ohio State University, 2Present address: Interdepartmental Neuroscience Graduate Program and Department of Neurobiology,Northwestern University, 3Quantitative Biology Institute,Ohio University, 4Department of Physics and Astronomy,Ohio University

Chapters

  • 00:04Introduction
  • 00:41Initial Assembly of Nerve Perfusion Chamber
  • 01:26Dissection and Preparation of Mouse Tibial Nerve
  • 03:09Final Nerve Perfusion Chamber Assembly
  • 04:48Fluorescence Activation and Image Acquisition
  • 06:21Flatfield and Darkfield Image Acquisition
  • 07:37Results: Pulse-escape and Pulse-spread Experiments
  • 09:17Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了荧光活化方法，用于分析来自转基因小鼠的周围神经单髓轴的轴感传输，这些轴感来自转基因小鼠，这些轴线来自表达可光激活神经丝蛋白的外周神经。

Tags

Neurofilament TransportFluorescence MicroscopyPhotoactivatable Fluorescent FusionMyelinated AxonsTibial NerveNerve DissectionPerfusion ChamberOxygenated SalineMicrodissectionNerve Imaging

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