JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biochemistry
/
大鼠胰腺组织快速、经济高效的RNA提取
JoVE Journal
Biochemistry
Author Produced
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biochemistry
Rapid and Cost-Effective RNA Extraction of Rat Pancreatic Tissue
Please note that all translations are automatically generated.
Click here for the English version.
大鼠胰腺组织快速、经济高效的RNA提取
DOI:
10.3791/61255-v
•
10:12 min
•
September 19, 2020
•
Sanaz Dastghaib
2
,
Zahra Shahsavar
,
Zahra Karimian
,
Pooneh Mokarram
5
1
Department of Biochemistry, School of Medicine
,
Shiraz University of Medical Sciences
,
2
Endocrinology and Metabolism Research Center, Nemazee Hospital
,
Shiraz University of Medical Sciences
,
3
English Language Department, Faculty of Paramedical Sciences
,
Shiraz University of Medical Sciences
,
4
Department of e-Learning in Medical Sciences, Virtual School and Center of Excellence in e-Learning
,
Shiraz University of Medical Sciences
,
5
Autophagy Research Center, School of Medicine
,
Shiraz University of Medical Sciences
Chapters
00:00
Introduction
00:58
The Removal of the Rat Pancreatic Tissue
03:33
RNA Extraction
08:00
Evaluating RNA Integrity with Denaturation Electrophoresis
08:40
Representative Results: The Evaluation of the RNA Integrity
09:50
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
分离的RNA的纯度和完整性是RNA依赖性测定中的重要一步。在这里,我们提出了一个实用、快速和廉价的方法,从少量未受损的胰腺组织中提取RNA。
Tags
RNA Extraction
Rapid Method
Cost-effective
Autolysis
RNA Stabilization
RNase Inactivation
RNA Integrity
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
从人体二尖瓣提取蛋白质的优化方案
小鼠脂肪组织的采集和 RNA 分析处理
哺乳动物基因翻译起始复合体形成的 Toeprinting 分析
HEK 细胞 Mitoribosome 的快速分离
利用单击化学辅助 rna-路特捕获 (无花果) 策略捕获和鉴定 rna 结合蛋白
台湾绿蜂胶的提取与分析
哺乳动物细胞周期内蛋白质激酶 rna 激活的 rna 相互作用研究
极快速和特殊的代谢标签的RNA在Vivo与4-Thiouracil (Ers4tU)
神经节苷脂提取、纯化和分析
光学镊子研究翻译调控中的RNA-蛋白质相互作用
Read Article