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Rapid and Cost-Effective RNA Extraction of Rat Pancreatic Tissue
JoVE Journal
Bioquímica
Author Produced
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JoVE Journal Bioquímica
Rapid and Cost-Effective RNA Extraction of Rat Pancreatic Tissue

Rapid and Cost-Effective RNA Extraction of Rat Pancreatic Tissue

DOI:

10:12 min

September 19, 2020

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Capítulos

  • 00:00Introduction
  • 00:58The Removal of the Rat Pancreatic Tissue
  • 03:33RNA Extraction
  • 08:00Evaluating RNA Integrity with Denaturation Electrophoresis
  • 08:40Representative Results: The Evaluation of the RNA Integrity
  • 09:50Conclusion

Summary

Tadução automática

The purity and integrity of the isolated RNA is a vital step in RNA dependent assays. Here, we present a practical, rapid, and inexpensive method to extract RNA from a small quantity of undamaged pancreatic tissue.

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