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ラット膵臓組織の迅速かつ費用対効果の高いRNA抽出

JoVE Journal
Biochemistry
Author Produced

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JoVE Journal Biochemistry

Rapid and Cost-Effective RNA Extraction of Rat Pancreatic Tissue

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ラット膵臓組織の迅速かつ費用対効果の高いRNA抽出

8,852 Views

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10:12 min

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September 19, 2020

DOI:

10.3791/61255-v

DOI:

10.3791/61255-v

•

10:12 min

September 19, 2020

•

9 Views

Sanaz Dastghaib^1,2, Zahra Shahsavar³, Zahra Karimian⁴, Pooneh Mokarram^1,5

¹Department of Biochemistry, School of Medicine,Shiraz University of Medical Sciences, ²Endocrinology and Metabolism Research Center, Nemazee Hospital,Shiraz University of Medical Sciences, ³English Language Department, Faculty of Paramedical Sciences,Shiraz University of Medical Sciences, ⁴Department of e-Learning in Medical Sciences, Virtual School and Center of Excellence in e-Learning,Shiraz University of Medical Sciences, ⁵Autophagy Research Center, School of Medicine,Shiraz University of Medical Sciences

Summary

単離されたRNAの純度と完全性は、RNA依存アッセイにおいて重要なステップです。ここでは、少量の損傷を受けていない膵臓組織からRNAを抽出する実用的で迅速かつ安価な方法を提示する。

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