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Gemethyleerde RNA ImmunoprecipatieTest voor studie m5C Modificatie in Arabidopsis
JoVE Journal
Genetics
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JoVE Journal Genetics
Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
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Gemethyleerde RNA ImmunoprecipatieTest voor studie m5C Modificatie in Arabidopsis

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6,170 Views

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08:50 min

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May 14, 2020

DOI:

10.3791/61231-v

DOI:
10.3791/61231-v

•

08:50 min
May 14, 2020

•

8 Views
, , ,
1Max Planck Institute of Molecular Plant Physiology, 2Department of Molecular Medicine, Medical School,University of Padua, 3Biologie de l’Ecole Normale Supérieure (IBENS), Paris, France

Summary

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De methylated RNA immunoprecipitation assay is een antilichaamgebaseerde methode die wordt gebruikt om te verrijken voor gemethyleerde RNA-fragmenten. In combinatie met diepe sequencing leidt het tot de identificatie van transcripties met de m5C-wijziging.

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Saplaoura, E., Perrera, V., Colot, V., Kragler, F. Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis. J. Vis. Exp. (159), e61231, doi:10.3791/61231 (2020).

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