ジャーナル
/
遺伝学
/
Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
JoVE Journal
遺伝学
This content is Free Access.
JoVE Journal 遺伝学
Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

6,129 Views

•

08:50 min

•

May 14, 2020

DOI:

10.3791/61231-v

DOI:
10.3791/61231-v

•

08:50 min
May 14, 2020

•

8 Views
, , ,
1Max Planck Institute of Molecular Plant Physiology, 2Department of Molecular Medicine, Medical School,University of Padua, 3Biologie de l’Ecole Normale Supérieure (IBENS), Paris, France

概要

Automatically generated

The methylated RNA immunoprecipitation assay is an antibody-based method used to enrich for methylated RNA fragments. Coupled with deep sequencing, it leads to the identification of transcripts carrying the m5C modification.

Explore More Videos

Methylated RNA ImmunoprecipitationMeRIPRNA ModificationTranscriptomeArabidopsisRNA ExtractionGuanidine ThiocyanateAcid Phenol1-bromo-3-chloropropaneIsopropanolSodium AcetateEthanolDNaseRNA Integrity NumberIn Vitro TranscriptSonication

Read Article

Cite this Article

Saplaoura, E., Perrera, V., Colot, V., Kragler, F. Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis. J. Vis. Exp. (159), e61231, doi:10.3791/61231 (2020).

Download .ris file

Copy

Share Video

.

Copy